Bikes are allowed on paved roads throughout Henry Horton State Park, including the Henry Horton Greenway. Bikes are not allowed on the trails, but the parks serves as the starting point for a variety of great road biking experiences.

Henry Horton State Park is host to a two-day 100+ mile bike ride each year that begins and ends at the park. This event is called the Horton 100 and offers cyclists to experience the best of what Middle Tennessee has to offer from the prime vantage point of a bike seat.